| Title |
Role of Hydrogen Generation by Klebsiella pneumoniae in the Oral Cavity |
| Author |
Tomoko Kanazuru1,2, Eisuke F. Sato1*, Kumiko Nagata3, Hiroshi Matsui2, Kunihiko Watanabe2, Emiko Kasahara1, Mika Jikumaru1, June Inoue4, and Masayasu Inoue1 |
| Address |
1Department of Biochemistry & Molecular Pathology, Osaka City University Medical School, Osaka 545-8585, Japan, 2Department of Applied Biochemistry, Kyoto Prefectural University, Kyoto 606-8522, Japan, 3Department of Food and Nutrition, Senri Kinran University, Shita 565-0873, Japan, 4Department of Gastroenterology, Kobe University Medical School, Kobe 650-0017, Japan |
| Bibliography |
Journal of Microbiology, 48(6),778-783, 2010,
|
| DOI |
|
| Key Words |
hydrogen, hydrogenase, oral cavity, K. pneumoniae |
| Abstract |
Some gastrointestinal bacteria synthesize hydrogen (H2) by fermentation. Despite the presence of bactericidal factors in human saliva, a large number of bacteria also live in the oral cavity. It has never been shown that oral bacteria also produce H2 or what role H2 might play in the oral cavity. It was found that a significant amount of H2 is synthesized in the oral cavity of healthy human subjects, and that its generation is
enhanced by the presence of glucose but inhibited by either teeth brushing or sterilization with povidone iodine. These observations suggest the presence of H2-generating bacteria in the oral cavity. The screening of commensal bacteria in the oral cavity revealed that a variety of anaerobic bacteria generate H2. Among them,
Klebsiella pneumoniae (K. pneumoniae) generated significantly large amounts of H2 in the presence of glucose. Biochemical analysis revealed that various proteins in K. pneumoniae are carbonylated under standard culture conditions, and that oxidative stress induced by the presence of Fe++ and H2O2 increases the number
of carbonylated proteins, particularly when their hydrogenase activity is inhibited by KCN. Inhibition of H2 generation markedly suppresses the growth of K. pneumoniae. These observations suggest that H2 generation and/or the reduction of oxidative stress is important for the survival and growth of K. pneumoniae in the oral
cavity. |
