| Title |
In Vitro Antiviral Activity of Red Alga, Polysiphonia morrowii Extract and Its Bromophenols Against Fish Pathogenic Infectious Hematopoietic Necrosis Virus and Infectious Pancreatic Necrosis Virus |
| Author |
Su-Yeun Kim, Seok Ryel Kim#, Myung-Joo Oh, Sung-Ju Jung, and So Young Kang* |
| Address |
Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea
#Present address: Pathology Division, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(1),102-106, 2011,
|
| DOI |
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| Key Words |
Polysiphonia morrowii, antiviral, fish pathogenic viruses, 3-bromo-4,5-dihydroxybenzylmethylether, 3-bromo-4,5-dihydroxybenzaldehyde |
| Abstract |
Our previous investigation revealed that 80% methanolic extract of the red alga Polysiphonia morrowii has significant antiviral activities against fish pathogenic viruses, infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV). The present study was conducted to identify compounds
attributed for its antiviral activities and investigate their antiviral activities against IHNV and IPNV. Activityguided fractionation for 80% methanolic extract of Polysiphonia morrowii using a cell-based assay measuring virus-induced cytopathic effect (CPE) on cells yielded a 90% methanolic fraction, which showed the highest antiviral activity against both viruses among fractions yielded from the extract. From the fraction, two bromophenols were isolated and identified as 3-bromo-4,5-dihydroxybenzyl methyl ether (1) and 3-bromo-4,5-dihydroxybenzaldehyde (2) based on spectroscopic analyses. For both compounds, the concentrations to inhibit 50% of flounder spleen cell (FSP cell) proliferation (CC50) and each viral replication (EC50) were measured. In the pretreatment test, 3-bromo-4,5-dihydroxybenzyl methyl ether (1) and 3-bromo-4,5-dihydroxybenzaldehyde
(2) exhibited significant antiviral activities showing selective index values (SI = CC50/EC50) of 20 to 42 against both IHNV and IPNV. In direct virucidal test, 3-bromo-4,5-dihydroxybenzyl methyl ether (1) showed significant antiviral activites against both viruses while 3-bromo-4,5-dihydroxybenzaldehyde (2) was significantly effective against only IHNV. Although antiviral efficacies of both compounds against
IHNV and IPNV were lower than those of ribavirin used as a positive control, our findings suggested that the red alga Polysiphonia morrowii and isolated two bromophenols may have potential as a therapeutic agent against fish viral diseases. |
