| Title |
Halomonas alkalitolerans sp. nov., a Novel Moderately Halophilic Bacterium Isolated from Soda Meadow Saline Soil in Daqing, China |
| Author |
Shuang Wang1, Qian Yang1*, Zhi-Hua Liu1, Lei Sun2, Dan Wei2, Jun-Zheng Zhang1, Jin-Zhu Song1, Yun Wang1, Jia Song1, Jin-Xia Fan1, Xian-Xin Meng2, and Wei Zhang2 |
| Address |
1Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, P. R. China, 2Soil Fertilizer and Environment Energy Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P. R. China |
| Bibliography |
Journal of Microbiology, 49(1),24-28, 2011,
|
| DOI |
|
| Key Words |
Halomonas alkalitolerans sp. nov., 16S rRNA gene sequence, fatty acid composition, DNA-DNA hybridization |
| Abstract |
A moderately halophilic bacterial strain 15-13T, which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13T were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13T
were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C18:1 ω7c (60.48%) and C16:0 (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13T
clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13T was Halomonas pantelleriensis DSM 9661T (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H. pantelleriensis DSM 9661T was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13T represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13T (=CGMCC 1.9129T =NBRC 106539T). |
