| Title |
NOTE] A Simple and Reliable Method for Obtaining Entomosporium Monoconidial Isolates |
| Author |
Mi-Jeong Park1, Quinn A. Holtslag2, and Hyeon-Dong Shin1* |
| Address |
1Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea, 2Greenspace Management, Civil Engineering Technology Department, Red River College, Manitoba, Canada |
| Bibliography |
Journal of Microbiology, 49(2),324-326, 2011,
|
| DOI |
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| Key Words |
Entomosporium mespili, fan-shaped colony, germination, isolation technique |
| Abstract |
Monoconidial isolates of Entomosporium mespili were successfully cultured using a simple isolation procedure. A detailed description of the steps required for isolating E. mespili is provided. The characteristic pattern of conidial germination and growth on potato dextrose agar is also described. The process that was successful
in obtaining pure isolates involved collecting material in the morning, picking a glistening white mass of conidia under dissecting microscope magnification, depositing a mass of conidia onto a drop of water on a glass slide, streaking the conidial mass onto potato dextrose agar, incubating the plate for 48 h, and
transferring the characteristic fan-shaped colonies to fresh medium. |
