| Title |
NOTE] Development of Porphyromonas gingivalis-Specific Quantitative Real-Time PCR Primers Based on the Nucleotide Sequence of rpoB |
| Author |
Soon-Nang Park1,2, Jae-Yoon Park3, and Joong-Ki Kook1,2* |
| Address |
1Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea, 2Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea, 3Department of Biochemistry, Chosun University, Gwangju 501-759, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(2),315-319, 2011,
|
| DOI |
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| Key Words |
Porphyromonas gingivalis, rpoB, species-specific, qPCR primers |
| Abstract |
Species-specific quantitative real-time PCR (qPCR) primers were developed for the detection of Porphyromonas gingivalis. These primers, Pg-F/Pg-R, were designed based on the nucleotide sequences of RNA polymerase β-subunit gene (rpoB). Species-specific amplicons were obtained from the tested P. gingivalis strains but not in any of the other strains (46 strains of 46 species). The qPCR primers could detect as little as 4 fg of P. gingivalis chromosomal DNA. These findings suggest that these qPCR primers are suitable for applications in epidemiological studies. |
