| Title |
NOTE] Improved Antibiotic Resistance Gene Cassette for Marker Exchange Mutagenesis in Ralstonia solanacearum and Burkholderia Species |
| Author |
Hae Young Um1, Eunsook Chung1, Jai-Heon Lee1, and Seon-Woo Lee1,2* |
| Address |
1Department of Medical Bioscience, Dong-A University, Busan 604-714, Republic of Korea, 2Department of Applied Biology, Dong-A University, Busan 604-714, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(2),305-308, 2011,
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| DOI |
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| Key Words |
antibiotic resistance, kanamycin, marker exchange, Ralstonia solanacearum, trimethoprim |
| Abstract |
Marker exchange mutagenesis is a fundamental approach to understanding gene function at a molecular level in bacteria. New plasmids carrying a kanamycin resistance gene or a trimethoprim resistance gene were constructed to provide antibiotic resistance cassettes for marker exchange mutagenesis in Ralstonia
solanacearum and many antibiotic-resistant Burkholderia spp. Insertion sequences present in the flanking sequences of the antibiotic resistance cassette were removed to prevent aberrant gene replacement and polar mutation during mutagenesis in wild-type bacteria. Plasmids provided in this study would be convenient for use in gene cassettes for gene replacement in other Gram-negative bacteria. |
