| Title |
NOTE] Identification of PhoB Binding Sites of the yibD and ytfK Promoter Regions in Escherichia coli |
| Author |
Yusuke Yoshida1, Shinichiro Sugiyama1, Tomoya Oyamada1, Katsushi Yokoyama1, Soo-Ki Kim2, and Kozo Makino1* |
| Address |
1Department of Applied Chemistry, National Defense Academy, Yokosuka 239-8686, Japan, 2Department of Animal Science and Environment, College of Animal Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(2),285-289, 2011,
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| DOI |
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| Key Words |
Escherichia coli, pho regulon, two-component regulatory system, yibD, ytfK |
| Abstract |
By using a lacZ operon fusion genomic library of the Escherichia coli O157:H7 Sakai, we identified phosphatestarvation-inducible (psi) promoters located upstream of the yibD and ytfK genes. They have been previously proposed to belong to the phosphate regulon (pho regulon) by Beak and Lee (2006), based on the DNA array and in vivo transcriptional experiments. However, the direct interaction of these promoters with the activator protein of the pho regulon, PhoB, has not been determined. We determined the binding regions of PhoB in these promoter regions by DNase I footprinting. Both regions contained two pho boxes similar to the consensus sequence for PhoB binding. |
