| Title |
Genome Sequence Analysis of H5N1 Influenza A Virus Isolated from a Vietnamese in 2007 |
| Author |
Dieu Linh Tran1, Kangmo Kim2,3, Jae Yoo Choi1, Hyun Dong Paik1, Si-Woo Choi4, Jin Yeul Ma5, Sung-Soon Kim2, Sung Joon Ahn3*, and Young Bong Kim1* |
| Address |
1College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Republic of Korea, 2Center for Immunology and Pathology, Korean National Institute of Health, Seoul 122-701, Republic of Korea, 3School of Information and Communication Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea, 4Department of Genetic Engineering, Faculty of Life Science and Technology, Sungkyunkwan University, Suwon 440-746, Republic of Korea, 5Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(2),274-279, 2011,
|
| DOI |
|
| Key Words |
avian influenza, H5N1, human infection, phylogenetic analysis, reassortment |
| Abstract |
Highly pathogenic H5N1 avian influenza A virus (AIV) crossed the species barrier and caused a number of deaths in humans in Vietnam and 14 other countries. Since the last report of human H5N1 infection in November 2005, the first documented H5N1 human infection was reported in June 2007 in Vietnam and was followed by 7 more cases, including 5 fatalities. In this study, we isolated and analyzed the full length of the H5N1 genome from a sample from the first patient in 2007. Phylogenetic analysis of eight genomic segments of the H5N1 virus strain (A/Vietnam/HN/2007, VNH07) revealed that this strain appears to be of genotype V and contains the HA gene, which is classified into clade 2.3.4. The deduced amino acid sequence of the HA protein has a typical affinity sequence for α2,3 linkage (SAα2,3-Gal) receptors
and typical multibasic cleavage sequences. Compared with other H5N1 isolates, VNH07 showed that the possible reassortments for the NA and NP segments occurred between A/goose/Guangxi/3017/2005-like isolates (2.3.2) and A/human/Zhejiang/16/2006-like isolates (2.3.4). |
