| Title |
Transcriptional Control of Genes Involved in Yeast Phospholipid Biosynthesis |
| Author |
Roshini Wimalarathna1,2, Chen-Han Tsai1, and Chang-Hui Shen1,2,3* |
| Address |
1Department of Biology, College of Staten Island, City University of New York, 2800 Victory Blvd, Staten Island, New York 10314, USA, 2PhD Program in Biology, The Graduate Center, City University of New York, 365 Fifth Avenue, New York 10016, USA, 3Institute for Macromolecular Assemblies, City University of New York, 2800 Victory Blvd, Staten Island, New York 10314, USA |
| Bibliography |
Journal of Microbiology, 49(2),265-273, 2011,
|
| DOI |
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| Key Words |
phospholipid biosynthesis, gene expression, UASINO, Ino2p, qRT-PCR |
| Abstract |
Phospholipid biosynthetic genes encode enzymes responsible for phospholipid biosynthesis. They are coordinately regulated by the availability of phospholipid precursors through the inositol-sensitive upstream activating sequence (UASINO). However, not all phospholipid genes are UASINO-containing genes and not all UASINO-containing genes have the same response to the phospholipid precursors. Therefore, the transcriptional regulation of phospholipid genes in response to the availability of phospholipid precursors is still unclear. Here, 22 out of 47 phospholipid biosynthetic genes were identified as UASINO-containing genes, including EKI1, EPT1, INM1, IPK2, KCS1, PAH1, and PIK1 which have never been reported before. We also showed, using qRTPCR technique, that 12 UASINO-containing genes are down-regulated by 100 μM inositol in the wild type cells and up-regulated by 100 μM inositol in the ino2Δ cells. Therefore, it is possible that these genes are transcriptionally regulated by the UASINO through the negative response of Ino2p to inositol. One other UASINO-containing gene might be regulated by the positive response of Ino2p to 100 μM inositol. Surprisingly, we found 9 UASINO-containing genes are not dependent on the response of Ino2p to 100 μM inositol, indicating that they may be regulated by other pathway. Furthermore, we identified 9 and 3 non-UASINO-containing genes that are possibly regulated by the negative and positive response of Ino2p to 100 μM inositol, respectively.
Therefore, these observations provide insight into the understanding of the co-regulated phospholipid biosynthetic genes expression. |
