| Title |
Functional Analysis of SGR4635-Induced Enhancement of Pigmented Antibiotic Production in Streptomyces lividans |
| Author |
Won-Jae Chi1, Soon-Youl Lee2, and JaeHag Lee3* |
| Address |
1Department of Biological Science, Myongji University, Gyeonggi-do 449-728, Republic of Korea, 2Department of Biotechnology, Hankyong National University, Gyeonggi-do 456-749, Republic of Korea, 3Department of Food and Nutrition, Seoil University, Seoul 131-702, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(5),828-833, 2011,
|
| DOI |
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| Key Words |
Streptomyces, antibiotic production, N-acetylglucosamine, transcriptional regulator, DasR |
| Abstract |
The Gram-positive mycelium-producing bacterium Streptomyces undergoes complex morphological differentiation after autolytic degradation of the vegetative mycelium. Cell-wall breakdown during growth stimulates cell development and secondary metabolite production by Streptomyces. N-acetylglucosamine (GlcNAc) produced by cell-wall lysis acts as a signal molecule, triggering the production of secondary metabolites in S. coelicolor A3(2). Here, we report that introduction of multiple copies of the GlcNAc-internalizing gene (sgr4635, encoding nagE2) of S. griseus activates actinorhodin and undecylprodigiosin production during the late growth of S. lividans in the absence of GlcNAc. Furthermore, the repressor-type transcriptional regulator DasR binds to two operator sites upstream of sgr4635. Our findings indicate that sgr4635 induces DasR-mediated antibiotic production by internalizing the GlcNAc accumulated from cell-wall lysis. |
