| Title |
Degradation of Endocrine Disrupting Chemicals by Genetic Transformants with Two Lignin Degrading Enzymes in Phlebia tremellosa |
| Author |
Hyunwoo Kum1, Sungsuk Lee2, Sunhwa Ryu2, and Hyoung T. Choi1* |
| Address |
1Molecular Microbiology Lab, Department of Biochemistry, Kangwon National University, Chunchon 200-701, Republic of Korea, 2Division of Forest Bioenergy, Korea Forest Research Institute, Seoul 130-712, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(5),824-827, 2011,
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| DOI |
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| Key Words |
bisphenol A, genetic transformation, nonylphenol, Phlebia tremellosa |
| Abstract |
A white rot fungus Phlebia tremellosa produced lignin degrading enzymes, which showed degrading activity against various recalcitrant compounds. However, manganese peroxidase (MnP) activity, one of lignin degrading enzymes, was very low in this fungus under various culture conditions. An expression vector that carried both the laccase and MnP genes was constructed using laccase genomic DNA of P. tremellosa and MnP cDNA from Polyporus brumalis. P. tremellosa was genetically transformed using the expression vector to obtain fungal transformants showing increased laccase and MnP activity. Many transformants showed highly increased laccase and MnP activity at the same time in liquid medium, and three of them were used to degrade endocrine disrupting chemicals. The transformant not only degraded bisphenol A and nonylphenol more rapidly but also removed the estrogenic activities of the chemicals faster than the wild type strain. |
