| Title |
Adaptive Stress Response to Menadione-Induced Oxidative Stress in Saccharomyces cerevisiae KNU5377 |
| Author |
Il-Sup Kim1*, Ho-Yong Sohn2, and Ingnyol Jin1* |
| Address |
1Department of Microbiology, Kyungpook National University, Daegu 702-701, Republic of Korea, 2Department of Food and Nutrition, Andong National University, Gyeongbuk 760-749, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(5),816-823, 2011,
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| DOI |
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| Key Words |
Saccharomyces cerevisiae KNU5377, menadione stress, adaptive response, cell rescue proteins, trehalose |
| Abstract |
The molecular mechanisms involved in the ability of yeast cells to adapt and respond to oxidative stress are of great interest to the pharmaceutical, medical, food, and fermentation industries. In this study, we investigated the time-dependent, cellular redox homeostasis ability to adapt to menadione-induced oxidative stress, using biochemical and proteomic approaches in Saccharomyces cerevisiae KNU5377. Time-dependent cell viability was inversely proportional to endogenous amounts of ROS measured by a fluorescence assay with 2′,7′-dichlorofluorescin diacetate (DCFHDA), and was hypersensitive when cells were exposed to the compound for 60 min. Morphological changes, protein oxidation and lipid peroxidation were also observed. To overcome the unfavorable conditions due to the presence of menadione, yeast cells activated a variety of cell rescue proteins including antioxidant enzymes, molecular chaperones, energy-generating metabolic enzymes, and antioxidant molecules such as trehalose. Thus, these results show that menadione causes ROS generation and high accumulation of cellular ROS levels, which affects cell viability and cell morphology and there is a correlation between resistance to menadione and the high induction of cell rescue proteins after cells enter into this physiological state, which provides a clue about the complex and dynamic stress response in yeast cells. |
