Journal of Microbiology

Title Rapid Discrimination of Potato Scab-Causing Streptomyces Species Based on the RNase P RNA Gene Sequences
Author Hang-Yeon Weon1, Jaekyeong Song1*, Byung-Yong Kim1, On-Suk Hur2, In-Cheol Park1, and Joo-Won Suh3
Address 1Agricultural Microbiology Team, National Academy of Agricultural Science, RDA, Suwon 441-707, Republic of Korea, 2Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Suwon 441-707, Republic of Korea, 3Department of Biological Science, Myong Ji University, Yong-in 449-728, Republic of Korea
Bibliography Journal of Microbiology, 49(5),791-796, 2011,
DOI
Key Words Streptomyces, potato scab, 16S rRNA, rnpB, phylogenetic analysis
Abstract Scab disease significantly damages potatoes and other root crops. Some Streptomyces species have been reported as potato-scab pathogens. Identification of the phytopathogenic Streptomyces is mainly done on the basis of the 16S rRNA gene, but use of this gene has some limitations for discriminating these strains because they share high similarities of 16S rRNA gene sequences. We tested the RNase P RNA (rnpB) gene as a taxonomic marker to clarify the relationship among closely related scab-causing Streptomyces strains. The rnpB genes were analyzed for 41 strains including 9 isolates from Jeju, Korea. There were 4 highly variable regions including nucleotide gaps in the rnpB genes. Interspecies similarity of the rnpB gene in tested Streptomyces strains was lower than about 97%, while the intraspecies similarity was higher than about 98%. Phylogenetic analysis demonstrated that the rnpB tree has similar topology to the 16S rRNA gene tree, but produces a more divergent phyletic lineage. These results revealed that the rnpB gene could be used as a powerful taxonomic tool for rapid differentiation of closely related Streptomyces species. In addition, it was also suggested that the variable regions marked as α, β, γ, and δ in the rnpB gene could be useful markers for the detection of specific Streptomyces species.
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