| Title |
NOTE] Molecular Characterization of Two Strains of Porcine Group C Rotavirus |
| Author |
Sung-Geun Lee1, Soo-Hyun Youn2, Mi-Hwa Oh3, Ok-Jae Rhee4, Sangsuk Oh2, and Soon-Young Paik1* |
| Address |
1Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea, 2Department of Food Science and Technology, College of Engineering, Ewha Womans University, Seoul 120-750, Republic of Korea, 3National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Republic of Korea, 4DK EcoV Environmental Microbiology Lab, Biotechnology Business Incubating Center, Dankook University, Chungnam 330-714, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(6),1058-1062, 2011,
|
| DOI |
|
| Key Words |
porcine group C rotavirus, South Korea, RT-PCR |
| Abstract |
Group C rotaviruses are an important cause of acute gastroenteritis in humans and animals. Fecal samples were collected from a porcine herd in July, 2009. Group C rotavirus RNA was detected using RT-PCR for the VP6 gene. The identified strain was further characterized by sequencing and phylogenetic analysis of the partial VP4, and complete VP6 and VP7 gene sequences. The partial VP4 and complete VP6 gene sequences of the CUK-5 strain were most closely related to those of the CUK-6 strain of group C rotaviruses. Phylogenetic analysis of the VP7 gene of the 2 strains (CUK-5 and CUK-6) and reference strains of group G rotavirus by the neighbor-joining method also confirmed that CUK-5 and CUK-6 belonged to type G5 and G1 strains, respectively. This study provides useful data for the prediction of newly appearing variants of porcine group C rotaviruses in neighboring countries through comparisons with GCRVs and fundamental research for vaccine development. |
