| Title |
NOTE] Bacillus manliponensis sp. nov., a New Member of the Bacillus cereus Group Isolated from Foreshore Tidal Flat Sediment |
| Author |
Min Young Jung1,2, Joong-Su Kim3, Woon Kee Paek4, Jeongheui Lim1, Hansoo Lee5, Pyoung Il Kim6, Jin Yeul Ma7, Wonyong Kim2, and Young-Hyo Chang1* |
| Address |
1Korean Collection for Type Cultures, Biological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea, 2Department of Microbiology, College of Medicine, Chung-Ang University, Seoul 156-756, Republic of Korea, 3Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeonbuk 580-185, Republic of Korea, 4National Science Museum, Daejeon 305-705, Republic of Korea, 5Korea Institute of Environmental Ecology, Daejeon 305-301, Republic of Korea, 6School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-744, Republic of Korea, 7Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(6),1027-1032, 2011,
|
| DOI |
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| Key Words |
Bacillus cereus group, Bacillus manliponensis sp. nov., phylogenetic, new species |
| Abstract |
A Gram-positive, endospore-forming, new Bacillus species, strain BL4-6T, was isolated from tidal flat sediment of the Yellow Sea. Strain BL4-6T is a straight rod, with motility by peritrichate flagella. The cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. The major fatty acids are iso-C15:0 and summed feature 3 (containing C16:1 ω7c/ iso-C15:0 2OH, and/or iso-C15:0 2OH/C16:1 ω7c). Cells are catalase-positive and oxidase-negative. The G+C content of the genomic DNA is 38.0 mol%. Based on a comparative 16S rRNA gene sequence analysis, the isolate belongs to the genus Bacillus, forms a clade with the Bacillus cereus group, and is closely related to Bacillus mycoides (98.5%), Bacillus cereus (98.5%), Bacillus anthracis (98.4%), Bacillus thuringiensis (98.4%), Bacillus weihenstephanensis (98.1%), and Bacillus pseudomycoides (97.5%). The isolate showed less than 85% similarity of the gyrA gene sequence and below 95% similarity of the rpoB gene sequence to the members of this group. DNA-DNA relatedness between strain BL4-6T and B. cereus group was found to be in a range of 22.8-42.3%, and thus BL4-6T represents a unique species. On the basis of these studies, strain BL4-6T (=KCTC 13319T =JCM 15802T) is proposed to represent the type strain of a novel species, Bacillus manliponensis sp. nov. |
