| Title |
Shift of Phylogenic Position in Megalocytiviruses Based on Three Different Genes |
| Author |
Se Ryun Kwon1, Toyohiko Nishizawa2*, Jong-Won Park3, and Myung-Joo Oh2 |
| Address |
1Department of Aquatic Life Medical Sciences, Sunmoon University, Chung Nam 336-708, Republic of Korea, 2Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea, 3Genetics & Breeding Research Center, National Fisheries Research & Development Institute, Geoje 656-842, Republic of Korea |
| Bibliography |
Journal of Microbiology, 49(6),981-986, 2011,
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| DOI |
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| Key Words |
Red seabream iridovirus, RSIVD, MCP gene, PstI fragment gene, phylogeny, megalocytivirus |
| Abstract |
Major capsid protein (MCP), the adenosine triphosphatase (ATPase), and the PstI fragment genes from five Japanese and three Korean megalocytivirus isolates were sequenced and phylogenetically analyzed with known megalocytiviruses. Phylogenetic trees formed three major clusters (M1, M2, and M3 or P1, P2, and P3), and genogroup I was divided into two minor clusters (M1a/M1b and P1a/P1b) using three target genes. Sequence identity was >97% within each cluster, except cluster II of the PstI fragment (>94% of sequence identity). Interestingly, different genotyping patterns were observed for the same isolates depending on the gene analyzed. The JPN-YelTail and JPN-BfTuna isolates located in the minor M1a cluster, based on MCP and ATPase nucleotide sequences, appeared in the minor P1b cluster based on the PstI fragment, suggesting a shift of phylogenic position in megalocytiviruses. Further study will be conducted to compare the viral antigenicity and pathogenicity between the two isolates showing the shift of phylogenic position and the other isolates clustered within genogroup I. |
