| Title |
Responses in the Expression of Extracellular Proteins in Methicillin-Resistant Staphylococcus aureus Treated with Rhodomyrtone |
| Author |
Monton Visutthi1, Potjanee Srimanote2, and Supayang Piyawan Voravuthikunchai1,3* |
| Address |
1Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand, 2Faculty of Allied Health Sciences, Thammasat University, Prathumthani 12120, Thailand, 3Natural Products Research Center, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand |
| Bibliography |
Journal of Microbiology, 49(6),956-964, 2011,
|
| DOI |
|
| Key Words |
Rhodomyrtus tomentosa, methicillin-resistant Staphylococcus aureus, rhodomyrtone, anti-MRSA, extracellular proteins |
| Abstract |
Rhodomyrtone from a medicinal plant species, Rhodomyrtus tomentosa, is a challenged effective agent against Gram-positive bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). The present study was undertaken to provide insight into MRSA extracellular protein expression following rhodomyrtone treatment. Secreteomic approach was performed on a representative clinical MRSA isolate exposing to subinhibitory concentration rhodomyrtone (0.174 μg/ml). The identified extracellular proteins of a response of MRSA to rhodomyrtone treated condition were both suppressed and overexpressed. Staphylococcal antigenic proteins, immunodominant antigen A (IsaA) and staphylococcal secretory antigen (SsaA) involved in cell wall hydrolysis were downregulated after the treatment. The results suggested that rhodomyrtone may interfere with WalK/WalR (YycG/YycF) system. Other enzymes such as lipase precursor and another lipase, glycerophosphoryl diester phosphodiesterase, were absent. In contrast, cytoplasmic proteins such as SpoVG and glycerol phosphate lipoteichoic acid synthase, and ribosomal proteins were found in the treated sample. Appearance of several cytoplasmic proteins in the treated culture supernatant revealed that the bacterial cell wall biosynthesis was disturbed. This finding provides a proteomic mapping of extracellular proteins after rhodomytone treatment. Extensive investigation is required for this natural compound as it has a great potency as an alternative anti-MRSA drug. |
