| Title |
Isolation and Identification of Lipopeptide Antibiotics from Paenibacillus elgii B69 with Inhibitory Activity Against Methicillin-Resistant Staphylococcus aureus |
| Author |
Rui Ding1, Xue-Chang Wu1*, Chao-Dong Qian1, Yi Teng1, Ou Li1, Zha-Jun Zhan2, and Yu-Hua Zhao1 |
| Address |
1Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, P. R. China, 2College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang Province, P. R. China |
| Bibliography |
Journal of Microbiology, 49(6),942-949, 2011,
|
| DOI |
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| Key Words |
Paenibacillus elgii, polypeptins, pelgipeptins, lipopeptide antibiotics |
| Abstract |
Two lipopeptide antibiotics, pelgipeptins C and D, were isolated from Paenibacillus elgii B69 strain. The molecular masses of the two compounds were both determined to be 1,086 Da. Mass-spectrometry, amino acid analysis and NMR spectroscopy indicated that pelgipeptin C was the same compound as BMY-28160, while pelgipeptin D was identified as a new antibiotic of the polypeptin family. These two peptides were active against all the tested microorganisms, including antibiotic-resistant pathogenic bacterial strains such as methicillin-resistant Staphylococcus aureus (MRSA). Time-kill assays demonstrated that pelgipeptin D exhibited rapid and effective bactericidal action against MRSA at 4×MIC. Based on acute toxicity test, the intraperitoneal LD50 value of pelgipeptin D was slightly higher than that of the structurally related antimicrobial agent polymyxin B. Pelgipeptins are highly potent antibacterial and antifungal agents, particularly against MRSA, and warrant further investigation as possible therapeutic agents for bacteria infections resistant to currently available antibiotics. |
