| Title |
Assessment of Resistomycin, as an Anticancer Compound Isolated and Characterized from Streptomyces aurantiacus AAA5 |
| Author |
Rajendran Vijayabharathi1*, Per Bruheim2, Trygve Andreassen2, Duraisamy Senthil Raja3, Palanisamy Bruntha Devi1, Sathyaseelan Sathyabama1, and Venkatesan Brindha Priyadarisini1 |
| Address |
1Department of Microbial Biotechnology, Bharathiar University, Coimbatore 641 046, India, 2Department of Biotechnology, Norwegian University of Science and Technology, Sem Selands vei 6/8, N-7491 Trondheim, Norway, 3Department of Chemistry, Bharathiar University, Coimbatore 641 046, India |
| Bibliography |
Journal of Microbiology, 49(6),920-926, 2011,
|
| DOI |
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| Key Words |
Streptomyces aurantiacus, resistomycin, cytotoxic compound, antibacterial activity |
| Abstract |
A new actinomycete strain, isolated from humus soils in the Western Ghats, was found to be an efficient pigment producer. The strain, designated AAA5, was identified as a putative Streptomyces aurantiacus strain based on cultural properties, morphology, carbon source utilization, and analysis of the 16S rRNA gene. The strain produced a reddish-brown pigmented compound during the secondary metabolites phase. A yellow compound was derived from the extracted pigment and was identified as the quinone-related antibiotic resistomycin based on ultraviolet–visible spectrophotometry, fourier transform infrared spectroscopy, liquid chromatography and mass spectroscopy, and nuclear magnetic resonance analyses. The AAA5 strain was found to produce large quantities of resistomycin (52.5 mg/L). It showed potent cytotoxic activity against cell lines viz. HepG2 (hepatic carcinoma) and HeLa (cervical carcinoma) in vitro, with growth inhibition (GI50) of 0.006 and 0.005 μg/ml, respectively. The strain also exhibited broad antimicrobial activities against both Gram-positive and Gram-negative bacteria. Therefore, AAA5 may have great potential as an industrial resistomycin-producing strain. |
