Title |
NOTE] Phycicoccus ochangensis sp. nov., Isolated from Soil of a Potato Cultivation Field |
Author |
Hyangmi Kim1,2, Hyun-Woo Oh3, Doo-Sang Park1, Kang Hyun Lee1, Sung Uk Kim3, Hee-Moon Park2, and Kyung Sook Bae1* |
Address |
1Microbiological Resource Center, KRIBB, Daejeon 305-806, Republic of Korea, 2Department of Microbiology & Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea, 3Industrial Bio-materials Research Center, KRIBB, Daejeon 305-806, Republic of Korea |
Bibliography |
Journal of Microbiology, 50(2),349-353, 2012,
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DOI |
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Key Words |
Phycicoccus ochangensis, potato field, Intrasporangiaceae |
Abstract |
Two novel, Gram-positive, motile, coccal bacteria, strains L1b-b9T and B5a-b5, were isolated from a potato cultivation field in Ochang, Korea. These isolates grew at 10–45°C, pH 5.0–10.0, and in the presence of 8% (w/v) NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was mesodiaminopimelic acid. The major menaquinone was MK-8(H4) and the main cellular fatty acids were iso-C14:0, iso-C15:0, and anteiso-C15:0. Polar lipids in strain L1b-b9T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unknown glyco-amino lipid. The G+C content of genomic DNA was 73.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains L1b-b9T and B5a-b5 shared 99.36% similarity and formed a robust clade with the type species of the genus Phycicoccus. Strain L1b-b9T is related most closely to Phycicoccus cremeus V2M29T (97.52% 16S rRNA gene sequence similarity). On the basis of phylogenetic characteristics, the name Phycicoccus ochangensis sp. nov. is proposed for strain LIb-b9T (=KCTC 19694T =JCM 17595T). |