| Title |
Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus |
| Author |
Choon-Mee Kim1, Seong-Jung Kim2, and Sung-Heui Shin1,3* |
| Address |
1Research Center for Resistant Cells, Chosun University Medical School, Gwangju 501-759, Republic of Korea, 2Department of Emergency Medicine, Chosun University Medical School, Gwangju 501-759, Republic of Korea, 3Department of Microbiology, Chosun University Medical School, Gwangju 501-759, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(2),320-325, 2012,
|
| DOI |
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| Key Words |
Vibrio vulnificus, iron, aerobactin, glucose, cyclic AMP-receptor protein, siderophore piracy |
| Abstract |
The ferrophilic bacterium Vibrio vulnificus can utilize the siderophore aerobactin of Escherichia coli for iron acquisition via its specific receptor IutA. This siderophore piracy by V. vulnificus may contribute to its survival and proliferation, especially in mixed bacterial environments. In this study, we examined the effects of glucose, cyclic AMP (cAMP), and cAMP-receptor protein (Crp) on iutA expression in V. vulnificus. Glucose dose-dependently repressed iutA expression. A mutation in cya encoding adenylate cyclase required for cAMP synthesis severely repressed iutA expression, and this change was recovered by in trans complementing cya or the addition of exogenous cAMP. Furthermore, a mutation in crp encoding Crp severely repressed iutA expression, and this change was recovered by complementing crp. Accordingly, glucose deprivation under iron-limited conditions is an environmental signal for iutA expression, and Crp functions as an activator that regulates iutA expression in response to glucose availability. |
