| Title |
Effect of Natural Mediators on the Stability of Trametes trogii Laccase during the Decolourization of Textile Wastewaters |
| Author |
Rim Khlifi-Slama1*, Tahar Mechichi2, Sami Sayadi1, and Abdelhafidh Dhouib1 |
| Address |
1Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sidi Mansour Road Km 6, PB 1177, 3018 Sfax, Tunisia, 2Ecole Nationale d’Ingénieurs de Sfax, Laboratory of Enzyme Engineering and Microbiology, University of Sfax, Soukra Road Km 4, BP 1173, 3038 Sfax, Tunisia |
| Bibliography |
Journal of Microbiology, 50(2),226-234, 2012,
|
| DOI |
|
| Key Words |
laccase, stability, natural mediator, wastewater |
| Abstract |
The purpose of the present study was to determine the
effect of natural mediators on the stability of the Trametes
trogii crude laccase in the process of decolourization of
textile effluents. Acetosyringone allowed the highest wastewaters
decolourization rate of 25%. At higher concentrations
of acetosyringone, the relative activity of laccase decreased
approximately by between 38% and 88% after 5 days of
incubation. T. trogii laccase was strongly inactivated at 3
mM syringaldehyde, after 3 days of incubation. However,
laccase activity is more stable in the presence of the vanillin
and m-coumarate. The T. trogii growth on solid effluentbased-
medium was examined and evaluated by measuring
the colony diameter in cm. T. trogii was completely inhibited
on 100:0 and 80:20 effluent:water solid medium, however,
colony diameter reached 5 cm on 60:40 effluent:water solid
medium after 13–14 days incubation. When the textile effluent
was pre-treated with laccase and laccase-acetosyringone
system, the colony diameter of 2 cm of T. trogii on 80:20
effluent:water solid medium was reached after 14 and 10
days of incubation respectively. |
