| Title |
Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Wheat Roots by Wheat Germ Agglutinin Labeled with Fluorescein Isothiocyanate |
| Author |
Jian Zhang1, Jingyang Liu1, Liyuan Meng1, Zhongyou Ma2, Xinyun Tang1*, Yuanyuan Cao1, and Leni Sun1 |
| Address |
1School of Life Science, Anhui Agricultural University, Hefei 230036 Anhui Province, P. R. China, 2Department of Biology, Anhui Science and Technology University, Bengbu 233100 Anhui Province, P. R. China |
| Bibliography |
Journal of Microbiology, 50(2),191-198, 2012,
|
| DOI |
|
| Key Words |
PGPR, FITC-labeled WGA, Bacillus sp. |
| Abstract |
Thirty-two isolates were obtained from wheat rhizosphere
by wheat germ agglutinin (WGA) labeled with fluorescein
isothiocyanate (FITC). Most isolates were able to produce
indole acetic acid (65.6%) and siderophores (59.3%), as well
as exhibited phosphate solubilization (96.8%). Fourteen isolates
displayed three plant growth-promoting traits. Among
these strains, two phosphate-dissolving ones, WS29 and
WS31, were evaluated for their beneficial effects on the early
growth of wheat (Triticum aestivum Wan33). Strain WS29
and WS31 significantly promoted the development of lateral
roots by 34.9% and 27.6%, as well as increased the root dry
weight by 25.0% and 25.6%, respectively, compared to those
of the control. Based on 16S rRNA gene sequence comparisons
and phylogenetic positions, both isolates were determined
to belong to the genus Bacillus. The proportion of
isolates showing the properties of plant growth-promoting
rhizobacteria (PGPR) was higher than in previous reports.
The efficiency of the isolation of PGPR strains was also
greatly increased by WGA labeled with FITC. The present
study indicated that WGA could be used as an effective tool
for isolating PGPR strains with high affinity to host plants
from wheat roots. The proposed approach could facilitate
research on biofertilizers or biocontrol agents. |
