| Title |
Identification and Enumeration of Microcystis Using a Sandwich Hybridization Assay |
| Author |
Jing Ping Zhu*, Xian Li, and Shi Du |
| Address |
School of Environmental Science and Engineering, Huazhong University of Science & Technology, Wuhan 430074, P. R. China |
| Bibliography |
Journal of Microbiology, 50(2),186-190, 2012,
|
| DOI |
|
| Key Words |
Microcystis, molecular probes, sandwich hybridization assay, PC-IGS |
| Abstract |
Based on sequence analyses of phycocyanin intergenic spacers
(PC-IGS) from Microcystis, Anabaena, Aphanizomenon,
and Planktothrix (Oscillatoria) strains, a genus-specific probe
pair TF/TR was designed, and a sandwich hybridization assay
was established to quantitatively detect Microcystis. Through
BLAST and cyanobacterial culture tests, TF/TR was demonstrated
to be specific for Microcystis. A calibration curve
for the sandwich hybridization assay was established, and
the lowest detected concentration was 100 cell/ml. Laboratory
and field samples were analyzed with both sandwich hybridization
assay and microscopy. The biotic and abiotic
components of the samples were of little disturbance to the
sandwich hybridization assay. The results showed no distinct
difference between the two methods. In this study, a sandwich
hybridization assay was established to detect Microcystis,
providing an alternative to traditional microscopic, morphology-
based methods. |
