| Title |
Effect of Salmonella Treatment on an Implanted Tumor (CT26) in a Mouse Model |
| Author |
Misun Yun1, SangO Pan2, Sheng- Nan Jiang3, Vu Hong Nguyen3, Seung-Hwan Park3, Che-Hun Jung4, Hyung-Seok Kim5, Jung-Joon Min3, Hyon E. Choy1, and Yeongjin Hong1* |
| Address |
1Departments of Microbiology, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, 2Jeollanamdo Institute of Natural Resources Research (JINR), Jeollanamdo 529-851, Republic of Korea, 3Departments of Nuclear Medicine, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, 4Departments of Chemistry, Chonnam National University, Gwangju 500-757, Republic of Korea, 5Forensic Medicine, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(3),502-510, 2012,
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| DOI |
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| Key Words |
Salmonella Typhimurium, bacterial tumor targeting, CT26, protein response |
| Abstract |
The use of bacteria has contributed to recent advances in targeted cancer therapy especially for its tumor-specific accumulation and proliferation. In this study, we investigated the molecular events following bacterial therapy using an attenuated Salmonella Typhimurium defective in ppGpp synthesis (ΔppGpp), by analyzing those proteins differentially expressed in tumor tissues from treated and untreated mice. CT26 murine colon cancer cells were implanted in BALB/c mice and allowed to form tumors. The tumor-bearing mice were treated with the attenuated Salmonella Typhimurium. Tumor tissues were analyzed by 2D-PAGE. Fourteen differentially expressed proteins were identified by mass spectrometry. The analysis revealed that cytoskeletal components, including vimentin, drebrin-like protein, and tropomyosinalpha 3, were decreased while serum proteins related to heme or iron metabolism, including transferrin, hemopexin, and haptoglobin were increased. Subsequent studies revealed that the decrease in cytoskeletal components occurred at the transcriptional level and that the increase in heme and iron metabolism proteins occurred in liver. Most interestingly, the same pattern of increased expression of transferrin, hemopexin, and haptoglobin was observed following radiotherapy at the dosage of 14 Gy. |
