| Title |
NOTE] Biosynthetic Pathway for Poly(3-Hydroxypropionate) in Recombinant Escherichia coli |
| Author |
Qi Wang1,2, Changshui Liu1,2, Mo Xian1, Yongguang Zhang1, and Guang Zhao1* |
| Address |
1Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, P. R. China, 2Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China |
| Bibliography |
Journal of Microbiology, 50(4),693-697, 2012,
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| DOI |
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| Key Words |
poly(3-hydroxypropionate), malonyl-CoA reductase, propionyl-coenzyme A synthetase, polyhydroxyalkanoate synthase, recombinant E. coli |
| Abstract |
Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexus aurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria. |
