| Title |
NOTE] The Activity of Phosphoinositide-Specific Phospholipase C Is Required for Vegetative Growth and Cell Wall Regeneration in Coprinopsis cinerea |
| Author |
Young Taek Oh, Chun-Seob Ahn, Kyung-Jin Lee, Jeong-Geun Kim, Hyeon-Su Ro, Jae Won Kim*, and Chang-Won Lee* |
| Address |
Department of Microbiology, College of Natural Science/ Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(4),689-692, 2012,
|
| DOI |
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| Key Words |
phosphoinositide-specific phospholipase C (PI-PLC), Coprinopsis cinerea, U73122 |
| Abstract |
Three isotypes of phosphoinositide-specific phospholipase C designated CcPLC1, CcPLC2, and CcPLC3 were identified in Coprinopsis cinerea, through a search of the genome sequence database. The functional role of the PI-PLCs were studied by using U73122, which specifically inhibits the activity of PI-PLC. The specificity of the inhibitor effect was confirmed by using an inactive structural analog U73433. The inhibition of PI-PLCs activity resulted in severely retarded germination of basidiospores and oidia, reduced hyphal growth, knobbly hyphal tips with many irregular side branches, and aberrant (branch-like structure) clamp cells. Furthermore, U73122 definitely inhibited cell wall formation. Here we report that PI-PLCs play important roles in various aspects of C. cinerea biology. |
