| Title |
NOTE] Fosmid Cloning, Nucleotide Sequence, and Characterization of a Beta-Lactamase Gene from Subsurface Isolates |
| Author |
Nurcan Vardar and Gönül Vardar-Schara* |
| Address |
Department of Genetics and Bioengineering, Fatih University, Büyükçekmece, Istanbul 34500, Turkey |
| Bibliography |
Journal of Microbiology, 50(4),680-683, 2012,
|
| DOI |
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| Key Words |
beta-lactamase, terrestrial subsurface, antibiotic resistance, direct cloning |
| Abstract |
A beta-lactamase gene was isolated for the first time from a terrestrial subsurface environment using a combined cultivation and direct cloning strategy. The gene, discovered from 24 m below land surface in Hawaii, was most similar to the penicillinase from Bacillus licheniformis. The resistance gene was confirmed via subcloning and its minimum inhibitory concentration values were measured against several test betalactam antibiotics. This study extends the knowledge on resistance to antimicrobials, which may help the efforts to minimize their future threat. |
