| Title |
NOTE] Probing the ArcA Regulon in the Rumen Bacterium Mannheimia succiniciproducens by Genome-Wide Expression Profiling |
| Author |
Seulgi Yun1,2, Jong Moon Shin1, Oh-Cheol Kim1, Young Ryul Jung1, Doo-Byoung Oh1,2, Sang Yup Lee3, and Ohsuk Kwon1,2* |
| Address |
1Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea, 2Biosystems and Bioengineering Program, University of Science and Technology, Daejeon 305-350, Republic of Korea, 3Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Center for Ultramicrochemical Process Systems, and Department of BioSystems, BioProcess Engineering Research Center and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(4),665-672, 2012,
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| DOI |
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| Key Words |
two-component signal transduction system, ArcA response regulator, target genes, Mannheimia succiniciproducens |
| Abstract |
In this study, the putative target genes of the Arc two-component system of the rumen bacterium Mannheimia succiniciproducens were determined by analyzing the transcriptome of the ArcA overexpression strain and by the in silico scanning of the entire genome sequence with the position weight matrix of the ArcA binding sequence developed for Escherichia coli. The majority of 79 repressed genes were involved in energy metabolism and carbohydrate transport and metabolism, while the majority of 82 induced genes were involved in hypothetical or unknown functions. Our results suggest that the Arc system in M. succiniciproducens has a specific function that differs from that in E. coli. |
