| Title |
Screening of Mutant Strain Streptomyces mediolani sp. AC37 for (-)-8-O-Methyltetrangomycin Production Enhancement |
| Author |
Jakeline Trejos Jiménez1*, Maria Sturdíková1, Vlasta Brezová2, Emil Svajdlenka3, and Marta Novotová4 |
| Address |
1Institute of Biotechnology and Food Science, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37 Bratislava, Slovak Republic, 2Institute of Physical Chemistry and Chemical Physics, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37 Bratislava, Slovak Republic, 3BEL/NOVAMANN International s.r.o, Komjatická 73, 940 02 Nové Zámky, Slovak Republic, 4Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlárska 5, SK-833 34 Bratislava, Slovak Republic |
| Bibliography |
Journal of Microbiology, 50(6),1014-1023, 2012,
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| DOI |
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| Key Words |
Streptomyces mediolani, (-)-8-O-methyltetrangomycin, mutation, N-nitroso-N-methyl-urea, EPR spectroscopy, sulphate radical anion, antioxidant |
| Abstract |
Streptomyces mediolani sp. AC37 was isolated from the root system of higher plant Taxus baccata and produced metabolite identified as (-)-8-O-methyltetrangomycin according to LC/MS/MS analysis. In our screening program for improvements
of bioactive secondary metabolites from plant associate streptomycetes, mutation was used as a tool for the induction of genetic variations for selection of higher (-)-8-O-methyltetrangomycin producers of isolates. S. mediolani sp. AC37 was treated with UV irradiation and chemical mutagenic treatment (N-nitroso-N-methyl-urea). The radical scavenging and antioxidant capacity of (-)-8-O-methyltetrangomycin
and extracts isolated from mutants were tested using EPR spin trapping technique and ABTS+ assay. Comparison of electron microscopic images of Streptomyces sp. AC37 and mutant strains of Streptomyces sp. AC37 revealed substantial differences in morphology and ultrastructure. |
