| Title |
A New Quorum-Sensing Inhibitor Attenuates Virulence and Decreases Antibiotic Resistance in Pseudomonas aeruginosa |
| Author |
Yu-Xiang Yang1, Zhen-Hua Xu1, Yu-Qian Zhang1, Jing Tian2, Li-Xing Weng1, and Lian-Hui Wang2* |
| Address |
1Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P. R. China, 2Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, Jiangsu 210046, P. R. China |
| Bibliography |
Journal of Microbiology, 50(6),987-993, 2012,
|
| DOI |
|
| Key Words |
Pseudomonas aeruginosa, quorum sensing, inhibitor, virulence factor, swarming, antimicrobial susceptibility |
| Abstract |
Quorum sensing (QS) has been a novel target for the treatment of infectious diseases. Here structural analogs of Pseudomonas aeruginosa autoinducer N-acyl homoserine lactone (AHL) were investigated for QS inhibitor (QSI) activity
and a novel QSI was discovered, N-decanoyl-L-homoserine benzyl ester (C2). Virulence assays showed that C2 downregulated total protease and elastase activities, as well as the production of rhamnolipid, that are controlled by QS in P.
aeruginosa wild-type strain PAO1 without affecting growth. C2 was also shown to inhibit swarming motility of PAO1. Using a microdilution checkerboard method, we identified synergistic interactions between C2 and several antibiotics, tobramycin, gentamycin, cefepime, and meropenem. Data from real-time RT-PCR suggested that C2 inhibited the expression of lasR (29.67%), lasI (21.57%), rhlR (28.20%), and
rhlI (29.03%). |
