| Title |
Genetic Organization and Conjugal Plasmid DNA Transfer of pHP69, a Plasmid from a Korean Isolate of Helicobacter pylori |
| Author |
Jung-Soo Joo1, Jae-Young Song2,3, Seung-Chul Baik2,3, Woo-Kon Lee2,3, Myung-Je Cho2,3, Kon-Ho Lee2,3, Hee-Shang Youn4, Ji-Hyun Seo4, Kwang-Ho Rhee2,3, and Hyung-Lyun Kang2,3* |
| Address |
1Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA, 2Department of Microbiology, Gyeongsang National University School of Medicine, Jinju 660-751, Republic of Korea, 3Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea, 4Department of Pediatrics, Gyeongsang National University School of Medicine, Jinju 660-751, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(6),955-961, 2012,
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| DOI |
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| Key Words |
Helicobacter pylori, pHP69, relaxase, conjugation |
| Abstract |
We isolated pHP69, a 9,153 bp plasmid from Helicobacter pylori with a 33.98% (G+C) content. We identified 11 open reading frames (ORFs), including replication initiation protein A (repA), fic (cAMP-induced filamentation protein), mccC, mccB, mobA, mobD, mobB, and mobC, as well as four 22 bp tandem repeat sequences. The nucleic acid and predicted amino acid sequences of these ORFs exhibited significant homology to those of other H. pylori plasmids. pHP69 repA encodes a replication initiation protein and its amino acid sequence is similar to those of replicase proteins from theta-type plasmids. pHP69 contains two types of repeat
sequences (R1 and R2), a MOBHEN family mobilization region comprising mobC, mobA, mobB, and mobD, and genes encoding microcin B and C. Among the 36 H. pylori strains containing plasmids, mobA or mccBC are present in 12 or 6, respectively and 3 contain both genes. To examine intrinsic capability of H. pylori for conjugative plasmid transfer, a shuttle vector pBHP69KH containing pHP69 and replication origin of pBR322 was constructed. It was shown that this vector could stably replicate and be mobilized among clinical H. pylori strains and demonstrated to gene transfer by natural plasmid. |
