| Title |
Fungal Community Associated with Genetically Modified Poplar During Metal Phytoremediation |
| Author |
Moonsuk Hur1, Young Woon Lim2, Jae Jeong Yu1, Se Uk Cheon1, Young Im Choi3, Seok-Hwan Yoon2, Sang-Cheol Park2, Dong-Il Kim4, and Hana Yi5* |
| Address |
1Nakdong River Environment Research Center, National Institute of Environmental Research, Goryeong-gum 717-873, Republic of Korea, 2School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea, 3Biotechnology Division, Korea Forest Research Institute, Suwon 441-350, Republic of Korea, 4Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea, 5Department of Environmental Health, Korea University, Seoul 136-703, Republic of Korea |
| Bibliography |
Journal of Microbiology, 50(6),910-915, 2012,
|
| DOI |
|
| Key Words |
fungal community, GMO, phytoremediation, metal contamination, NGS |
| Abstract |
Due to the increasing demand for phytoremediation, many transgenic poplars have been developed to enhance the bioremediation of heavy metals. However, structural changes to indigenous fungal communities by genetically modified organisms (GMO) presents a major ecological issue, due to the important role of fungi for plant growth in natural environments. To evaluate the effect of GM plant use on environmental fungal soil communities, extensive sequencing-based community analysis was conducted, while controlling the influence of plant clonality, plant age, soil condition, and harvesting season. The rhizosphere soils of GM and wild
type (WT) poplars at a range of growth stages were sampled together with unplanted, contaminated soil, and the fungal community structures were investigated by pyrosequencing the D1/D2 region of the 28S rRNA gene. The results show
that the overall structure of the rhizosphere fungal community was not significantly influenced by GM poplars. However, the presence of GM specific taxa, and faster rate of community change during poplar growth, appeared to be characteristic of the GM plant-induced effects on soil-born fungal communities. The results of this study provide additional information about the potential effects of GM poplar trees aged
1.5–3 years, on the soil fungal community. |
