| Title | Bhargavaea indica sp. nov., a Member of the Phylum Firmicutes, Isolated from Arabian Sea Sediment |
|---|---|
| Author | Pankaj Verma1, Chi Nam Seong2*, Prashant Kumar Pandey1, Ramesh Ramchandra Bhonde3, Cathrin Spröer4, Manfred Rohde5, and Yogesh Shreepad Shouche1,6* |
| Address | 1Molecular Biology Unit, National Centre for Cell Science, Pune 411 007, Maharashtra, India, 2Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea, 3Manipal Institute of Regenerative Medicine, Bangalore 560 071, Karnataka, India, 4Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany, 5Helmholtz-Zentrum für Infektionsforschung GmbH, D-38124 Braunschweig, Germany, 6Microbial Culture Collection, National Centre for Cell Science, Pune 411 007, Maharashtra, India |
| Bibliography | Journal of Microbiology, 51(1),36-42, 2013, |
| DOI | 10.1007/s12275-013-2488-z |
| Key Words | Bhargavaea indica, Firmicutes, 16S rRNA gene, gyrB |
| Abstract | A Gram-positive, aerobic, coccoid-rod shaped, non-motile, catalase- and oxidase-positive bacterium, designated strain KJW98T, was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic, nonendospore-forming and grew with 0–8.5% (w/v) NaCl, at 15–48°C and at pH 6.5–9.0, with optimum growth with 0.5% (w/v) NaCl, at 42°C and at pH 7.0–8.0. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain KJW98T forms a lineage within the genus Bhargavaea. The G+C content of the genomic DNA was 55 mol%. The DNA–DNA relatedness values of strain KJW98T with B. eijingensis DSM 19037T, B. cecembensis LMG 24411T and B. ginsengi DSM 19038T were 43.2, 39 and 26.5%, respectively. The major fatty acids were anteiso-C15:0 (37.7%), iso-C15:0 (19.7%), anteiso-C17:0 (17.0%) and iso-C16:0 (11.1%). The predominant menaquinone was MK-8 and the cell-wall peptidoglycan was of A4α type with L-lysine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The phenotypic, genotypic and DNA–DNA relatedness data indicate that strain KJW98T should be distinguished from the members of the genus Bhargavaea, for which the name Bhargavaea indica sp. nov. is proposed with the type strain KJW98T (=KCTC 13583T =LMG 25219T). |