| Title | NOTE] Paraherbaspirillum soli gen. nov., sp. nov. Isolated from Soil |
|---|---|
| Author | Rangasamy Anandham1, Soo-Jin Kim2, Ji Young Moon2, Hang-Yeon Weon2, and Soon-Wo Kwon2* |
| Address | 1Department of Agricultural Microbiology, Agricultural College and Research Institute, Madurai, India, 2Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea |
| Bibliography | Journal of Microbiology, 51(2),262-267, 2013, |
| DOI | 10.1007/s12275-013-2563-5 |
| Key Words | DNA-DNA hybridization, Paraherbaspirillum soli, taxonomy, 16S rRNA gene sequence |
| Abstract | A bacterial strain, designated JS5-2T, was isolated from soil collected from Jeju Island, Republic of Korea. The cells of the strain were Gram-negative, nonspore forming, catalaseand oxidase-positive, aerobic, nonmotile and rod-shaped. Strain JS5-2T exhibited 96.2–97.2, 95.1–96.3, and 95.4–95.8% 16S rRNA gene sequence similarities to the genera Herbaspirillum, Oxalicibacterium, and Herminiimonas, respectively. The highest sequence similarities were with Herbaspirillum autotrophicum IAM 14942T (97.2%) and Herbaspirillum frisingense GSF30T (97.1%). The major fatty acids of strain JS5-2T were C16:0 (35.0%), C17:0 cyclo (19.9%), C18:1 ω7c (11.4%), and summed feature 3 (C16:1 ω7c/C15:0 iso 2-OH) (15.2%), and the major polar lipids of strain JS5-2T were diphosphatidylglycerol and an unknown aminophospholipid. The strain contained Q-8 as the predominant ubiquinone. DNA-DNA relatedness values between strain JS5-2T and H. autotrophicum IAM 14942T, and H. frisingense GSF30T were 32 and 35%, respectively. The DNA G+C content of strain JS5-2T was 59.0 mol%. On the basis of phenotypic, genotypic, and physiological evidence, strain JS5-2T represents a novel species of a new genus, for which the name Paraherbaspirillum soli gen. nov., sp. nov. is proposed. The type strain JS5-2T (=KACC 12633T =NBRC 106496T) is proposed. |