| Title | NOTE] Rhodanobacter umsongensis sp. nov., Isolated from a Korean Ginseng Field |
|---|---|
| Author | Yi-Seul Kim1, Soo-Jin Kim1, Rangasamy Anandham2, Hang-Yeon Weon1, and Soon-Wo Kwon1* |
| Address | 1Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea, 2Department of Agricultural Microbiology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, Tamil Nadu, India |
| Bibliography | Journal of Microbiology, 51(2),258-261, 2013, |
| DOI | 10.1007/s12275-013-2516-z |
| Key Words | Rhodanobacter umsongensis, 16S rRNA gene sequence, taxonomy |
| Abstract | A bacterial isolate designated GR24-2T was isolated from Korean soil used for cultivating ginseng (Panax ginseng C. A. Meyer). The strain was aerobic, Gram-negative, motile, and rod-shaped. It grew optimally at 28–30°C, pH 7.0, and in a range of 0–1% NaCl. Phylogenetically, the strain clustered with members of the genus Rhodanobacter. The strain exhibited the highest sequence similarities (>98%) with R. panaciterrae LnR5-47T (98.4%), R. soli DCY45T (98.2%), and R. ginsengisoli GR17-7T (98.0%). However, it also showed high sequence similarities (>97%) with some other Rhodanobacter and Dyella species. The strain contained Q-8 as the predominant respiratory quinone. The major fatty acids (greater than 10% of the total fatty acids) were iso-C17:1 ω9c (24.5%), iso-C16:0 (22.8%), anteiso-C15:0 (10.5%), and iso-C15:0 (10.1%). Its major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unknown aminophospholipid. The DNA G+C content of strain GR24-2T was 65.6 mol%. The strain showed less than 70% DNA relatedness values between the closely related Rhodanobacter and Dyella species. The phylogeny, phenotype, DNA–DNA hybridization, and chemotaxonomic data generated in this study reveal that the isolate is a novel species of the genus Rhodanobacter. The name proposed for this strain is Rhodanobacter umsongensis sp. nov. (type strain GR24-2T =KACC 12917T =DSM 21300T). |