| Title | Construction of a Streptomyces lydicus A01 Transformant with a chit42 Gene from Trichoderma harzianum P1 and Evaluation of Its Biocontrol Activity against Botrytis cinerea |
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| Author | Qiong Wu1, Linquan Bai2, Weicheng Liu3, Yingying Li1, Caige Lu3, Yaqian Li1, Kehe Fu1, Chuanjin Yu1, and Jie Chen1* |
| Address | 1School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China, 2School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, P. R. China, 3Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P. R. China |
| Bibliography | Journal of Microbiology, 51(2),166-173, 2013, |
| DOI | 10.1007/s12275-013-2321-8 |
| Key Words | Streptomyces lydicus A01, chit42 from Trichoderma harzianum P1, Botrytis cinerea, chitinase activity, natamycin production |
| Abstract | Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea. To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea, this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01-chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01-chit42 CT on B. cinerea, including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma, which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens. |