| Title | NOTE] Lysinibacillus chungkukjangi sp. nov., Isolated from Chungkukjang, Korean Fermented Soybean Food |
|---|---|
| Author | Soo-Jin Kim1, Yun-Hee Jang1, Moriyuki Hamada2, Jae-Hyung Ahn1, Hang-Yeon Weon1, Ken-ichiro Suzuki2, Kyung-Sook Whang3, and Soon-Wo Kwon1* |
| Address | 1Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea , 2NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan, 3Institute of Microbial Ecology and Resources, Mokwon University, Daejeon 302-729, Republic of Korea |
| Bibliography | Journal of Microbiology, 51(3),400-404, 2013, |
| DOI | 10.1007/s12275-013-2664-1 |
| Key Words | DNA-DNA hybridization, Lysinibacillus chungkukjangi, taxonomy, 16S rRNA |
| Abstract | One bacterial strain 2RL3-2T was isolated from Chungkukjang, a traditional Korean fermented food made from soybeans, and determined to be a Gram-positive, aerobic, sporeforming rod. Growth of the novel strain was optimal at 30°C and pH 7.0. The 16S rRNA gene of strain 2RL3-2T showed the highest level of sequence similarity to Lysinibacillus sinduriensis BLB-1T (99.0%), Lysinibacillus massiliensis 4400831T (97.1%), Lysinibacillus xylanilyticus XDB9T (97.0%), and Lysinibacillus odysseyi 34hs-1T (96.8%). Phylogenetic analysis showed that strain 2RL3-2T formed a robust cluster with L. sinduriensis BLB-1T, L. massiliensis 4400831T, and L. odyssey 34hs-1T. The major fatty acids were anteiso-C15:0 (47.3%), iso-C16:0 (16.3%), and anteiso-C17:0 (11.3%), and the only menaquinone was MK-7. Diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine were the major polar lipids, along with an unknown phospholipid and two unknown lipids. The peptidoglycan type was A4α, with an interpeptide bridge of l-Lys–d-Asp. DNA-DNA hybridization values between strain 2RL3-2T and closely related Lysinibacillus species were below 43±4%. Therefore, based on phenotypic, chemotaxonomic, and phylogenetic characteristics, it was determined that strain 2RL3-2T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillus chungkukjangi sp. nov. is proposed. The type strain is 2RL3-2T (=KACC 16626T =NBRC 108948T). |