| Title | Paenibacillus marinisediminis sp. nov., a Bacterium Isolated from Marine Sediment |
|---|---|
| Author | Hae-Won Lee1,2, Seong Woon Roh1, Kyung June Yim2, Na-Ri Shin1, Jina Lee1, Tae Woong Whon1, Joon Yong Kim1, Dong-Wook Hyun1, Daekyung Kim2, and Jin-Woo Bae1* |
| Address | 1Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea, 2Jeju Center, Korea Basic Science Institute, Jeju 690-756, Republic of Korea |
| Bibliography | Journal of Microbiology, 51(3),312-317, 2013, |
| DOI | 10.1007/s12275-013-3198-2 |
| Key Words | bacterial taxonomy, marine sediment, Paenibacillus marinisediminis sp. nov. |
| Abstract | A Gram-negative, nonmotile, endospore-forming, rod-shaped bacterial strain LHW35T, which belonged to the genus Paenibacillus, was isolated from marine sediment collected from the south coast of the Republic of Korea. A phylogenetic analysis of 16S rRNA gene sequences indicated that strain LHW35T was most closely related to Paenibacillus taiwanensis G-soil-2-3T (97.2% similarity). The optimal growth conditions for strain LHW35T were 37°C, pH 6.0, and 0% (w/v) NaCl. The main isoprenoid quinone was menaquinone-7 (MK-7) and the major polyamine was spermidine. The diamino acid present in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major fatty acids were anteiso-C15:0 and C16:0. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, unidentified aminohospholipids, unidentified phospholipids, and unidentified polar lipids. A DNA-DNA hybridization experiment using the type strain of P. taiwanensis indicated <40% relatedness. The DNA G+C content was 45.0 mol%. Based on these phylogenetic, genomic, and phenotypic analyses, strain LHW35T should be classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus marinisediminis sp. nov. is proposed. The type strain is LHW35T (=KACC 16317T =JCM 17886T). |