Title NOTE] Anaerobic Cellulolytic Rumen Fungal Populations in Goats Fed with and without Leucaena leucocephala Hybrid, as Determined by Real-Time PCR
Author Ching Mun Kok1, Chin Chin Sieo1,2, Hui Yin Tan1, Wan Zuhainis Saad2, Juan Boo Liang3, and Yin Wan Ho1*
Address 1Institute of Bioscience, Universiti Putra Malaysia, Selangor, 43400, Malaysia, 2Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400, Malaysia, 3Institute of Tropical Agriculture, Universiti Putra Malaysia, Selangor, 43400, Malaysia
Bibliography Journal of Microbiology, 51(5),700-703, 2013,
DOI 10.1007/s12275-013-2540-z
Key Words Leucaena, condensed tannins, rumen fungal population, real-time PCR
Abstract The effect of Leucaena leucocephala hybrid-Bahru (LLB), which contains a high concentration of condensed tannins, on cellulolytic rumen fungal population in goats was investigated using real-time PCR. The fungal population in goats fed LLB was inhibited during the first 10 days of feeding, but after 15 days of feeding, there was a tremendous increase of fungal population (157.0 μg/ml), which was about fourfold more than that in control goats (39.7 μg/ml). However, after this period, the fungal population decreased continuously, and at 30 days of feeding, the fungal population (50.6 μg/ml) was not significantly different from that in control goats (55.4 μg/ml).