Title NOTE] Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain
Author Hong-Il Kim1, Jae-Young Nam1, Jae-Yong Cho2, Chang-Soo Lee1, and Young-Jin Park1*
Address 1Department of Biomedical Chemistry, Konkuk University, Chung-Ju 380-701, Republic of Korea, 2Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Republic of Korea
Bibliography Journal of Microbiology, 51(6),877-880, 2013,
DOI 10.1007/s12275-013-3236-0
Key Words transcriptome, mutation, genome, Corynebacterium glutamicum, RT-PCR
Abstract In the present study, 151 genes showed a significant change in their expression levels in Corynebacterium glutamicum ATCC 21300 compared with those of C. glutamicum ATCC 13032. Of these 151 genes, 56 genes (2%) were up-regulated and 95 genes (3%) were down-regulated. RNA sequencing analysis also revealed that 11 genes, involved in the L-lysine biosynthetic pathway of C. glutamicum, were up- or downregulated compared with those of C. glutamicum ATCC 13032. Of the 151 genes, 10 genes were identified to have mutations including SNP (9 genes) and InDel (1 gene). This information will be useful for genome breeding of C. glutamicum to develop an industrial amino acid-producing strain with minimal mutation.