Title |
NOTE] Lysinibacillus jejuensis sp. nov., Isolated from Swinery Waste |
Author |
Ji-Young Kim1,2, So-Hyun Park2, Duck-Chul Oh1*, and Young-Ju Kim2* |
Address |
1Department of Biology, College of Natural Science, Jeju National University, Jeju 690-756, Republic of Korea, 2Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 699-943, Republic of Korea |
Bibliography |
Journal of Microbiology, 51(6),872–876, 2013,
|
DOI |
10.1007/s12275-013-2500-7
|
Key Words |
Firmicutes, Lysinibacillus jejuensis sp. nov., swinery waste |
Abstract |
A Gram-positive, endospore-forming, rod-shaped bacterium, designated strain N2-5T, was isolated from swinery waste collected in Jeju, Republic of Korea. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated
that strain N2-5T formed a phyletic group within the phylum
Firmicutes with less than 97.0% similarities to members of
the genus Lysinibacillus, its nearest phylogenetic neighbors.
The highest levels of sequence similarity to the isolate were
observed against Lysinibacillus xylanilyticus XDB9T (96.8%),
Lysinibacillus macroides LMG 18474T (95.6%), and Lysinibacillus
parviboronicapiens BAM-582 T (95.6%). The organism
grew optimally at 30°C and pH 7 and in the presence of 1–3%
(w/v) NaCl. Strain N2-5T was chemotaxonomically characterized
by possessing menaquinone-7 (MK-7) as the major
menaquinone, and iso-C15:0 (54.9%), iso-C17:1ω10c (12.0%),
and C16:1ω7c alcohol (11.8%) as the predominant fatty acids.
The genomic DNA G+C content of the novel strain was
43.3 mol% and the cell-wall peptidoglycan was type A4α.
The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol,
and phosphatidylethanolamine. Based on
its phenotypic properties and phylogenetic data, strain N2-5T
(=DSM 28310T =KCTC13837 T) represents a novel species in
the genus Lysinibacillus, for which the name Lysinibacillus
jejuensis sp. nov. is proposed. |