Title |
Evaluation of Bakanae Disease Progression Caused by Fusarium fujikuroi in Oryza sativa L. |
Author |
In Sun Hwang1, Woo-Ri Kang1, Duk-Ju Hwang1, Shin-Chul Bae1, Sung-Hwan Yun2, and Il-Pyung Ahn1* |
Address |
1National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea, 2Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Republic of Korea |
Bibliography |
Journal of Microbiology, 51(6),858–865, 2013,
|
DOI |
10.1007/s12275-013-3472-3
|
Key Words |
Bakanae disease, Taqman real time PCR |
Abstract |
Bakanae disease caused by Fusarium fujikuroi is an important
fungal disease in rice. Among the seven strains isolated from
symptomatic rice grains in this study, one strain, FfB14,
triggered severe root growth inhibition and decay in the
crown and root of rice seedlings. The remaining six strains
caused typical Bakanae symptoms such as etiolation and
abnormal succulent rice growth. To reveal the relationship
between mycelial growth in the infected tissues and Bakanae
disease progression, we have established a reliable quantification
method using real time PCR that employs a primer
pair and dual-labeled probe specific to a unigene encoding
F. fujikuroi PNG1 (FfPNG1), which is located upstream of
the fumonisin biosynthesis gene cluster. Plotting the crossing
point (CP) values from the infected tissue DNAs on a standard
curve revealed the active fungal growth of FfB14 in
the root and crown of rice seedlings, while the growth rate
of FfB20 in rice was more than 4 times lower than FfB14.
Massive infective mycelial growth of FfB14 was evident in
rice stems and crown; however, FfB20 did not exhibit vigorous
growth. Our quantitative evaluation system is applicable
for the identification of fungal virulence factors other
than gibberellin. |