| Title | Inhibitory Effect of Chlorophyllin on the Propionibacterium acnes-Induced Chemokine Expression |
|---|---|
| Author | Mi-Sun Kang1, Jin-Hee Kim1, Boo-Ahn Shin1, Hyun-Chul Lee1, Youn-Shin Kim2, Hae-Soon Lim3, and Jong-Suk Oh1* |
| Address | 1Department of Microbiology, School of Medicine, Chonnam National University, Gwangju 501-746, Republic of Korea, 2Department of Forensic Medicine, School of Medicine, Chosun University, Gwangju 501-759, Republic of Korea, 3Department of Dental Education, School of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea |
| Bibliography | Journal of Microbiology, 51(6),844–849, 2013, |
| DOI | 10.1007/s12275-013-3015-y |
| Key Words | chlorophyllin, interleukin-8, monocyte chemotactic protein 1, Propionibacterium acnes, THP-1 cells |
| Abstract | Chlorophyllin (CHL), a chlorophyll-derivative, exhibits several beneficial properties, including antibacterial, antioxidant, and anticancer activities. However, its antibacterial and anti-inflammatory activities against Propionibacterium acnes have not been described. The antibacterial activity of this compound was evaluated in vitro using the broth microdilution method. CHL had an inhibitory effect on the growth of P. acnes (MIC = 100 μM). In a real-time reverse transcriptionpolymerase chain reaction and an enzyme-linked immunosorbent assay, CHL significantly decreased interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) production in a dose-dependent manner, decreasing both mRNA and protein levels for these chemokines in THP-1 cells indicating the anti-inflammatory effects of it. To investigate the molecular mechanisms underlying the anti-inflammatory properties of CHL in THP-1 cells stimulated by P. acnes, we used western blotting to analyze the effect of CHL on activation of the nuclear factor (NF)-κB. CHL inhibited P. acnes-induced IL-8 and MCP-1 production via blockade of NF-κB activation in THP-1 cells. Therefore, based on these results, we suggest that CHL is a useful agent to control the growth of P. acnes involved in acne inflammation and prevent acne. |