Title Cyclic Dipeptides from Lactic Acid Bacteria Inhibit Proliferation of the Influenza A Virus
Author Min-Kyu Kwak1, Rui Liu1, Jun-Oh Kwon1, Min-Kyu Kim2, Andrew HyoungJin Kim1, and Sa-Ouk Kang1*
Address 1Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-747, Republic of Korea, 2Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea
Bibliography Journal of Microbiology, 51(6),836–843, 2013,
DOI 10.1007/s12275-013-3521-y
Key Words Lactobacillus plantarum LBP-K10, culture filtrate, cis-cyclo(L-Leu-L-Pro), cis-cyclo(L-Phe-L-Pro), influenza A virus, antiviral activity
Abstract We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp., and Lactococcus lactis.