Title Reduction of Organic and Inorganic Selenium Compounds by the Edible Medicinal Basidiomycete Lentinula edodes and the Accumulation of Elemental Selenium Nanoparticles in Its Mycelium
Author Elena Vetchinkina, Ekaterina Loshchinina*, Viktor Kursky, and Valentina Nikitina
Address Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russian Federation
Bibliography Journal of Microbiology, 51(6),829–835, 2013,
DOI 10.1007/s12275-013-2689-5
Key Words shiitake, elemental selenium, nanospheres, intracellular synthesis
Abstract We report for the first time that the medicinal basidiomycete Lentinula edodes can reduce selenium from inorganic sodium selenite (SeIV) and the organoselenium compound 1,5- diphenyl-3-selenopentanedione-1,5 (DAPS-25) to the elemental state, forming spherical nanoparticles. Submerged cultivation of the fungus with sodium selenite or with DAPS- 25 produced an intense red coloration of L. edodes mycelial hyphae, indicating accumulation of elemental selenium (Se0) in a red modification. Several methods, including transmission electron microscopy (TEM), electron energy loss spectroscopy (EELS), and X-ray fluorescence, were used to show that red Se0 accumulated intracellularly in the fungal hyphae as electron-dense nanoparticles with a diameter of 180.51±16.82 nm. Under designated cultivation conditions, shiitake did not reduce selenium from sodium selenate (SeVI).