Title Lactobacillus paracasei subsp. paracasei LC01 Positively Modulates Intestinal Microflora in Healthy Young Adults
Author Hao Zhang1,2, Jing Sun1, Xianting Liu1, Chuan Hong1, Yuanbo Zhu1, Aiping Liu3, Siqi Li1, Huiyuan Guo1, and Fazheng Ren1,2*
Address 1Beijing Laboratory for Food Quality and Safety, and Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, P. R. China, 2Beijing Higher Institution Engineering Research Center of Animal Product, Beijing, P. R. China, 3Diary Department, MengNiu Diary Company, Beijing, P. R. China
Bibliography Journal of Microbiology, 51(6),777–782, 2013,
DOI 10.1007/s12275-013-3279-2
Key Words human trial, Lactobacillus paracasei subsp. paracasei LC01, intestinal microflora, short-chain fatty acid, ammonia
Abstract Lactobacillus paracasei subsp. paracasei LC01 (LC01) can tolerate intestinal stresses and has antioxidant activity. To evaluate the effect of the bacterium on human intestinal microflora, a randomized, double-blind, placebo-controlled human trial was carried out. Fifty-two healthy adult volunteers were randomized equally to two groups. One group consumed 12% (wt/vol) skimmed milk supplemented with 1010 CFU of LC01 each day for the 4-week treatment period, and then consumed placebo in the next treatment period, separated by a 2-week washout. The other group followed the reverse order. Group-specific real-time PCR and biochemical analyses was used to determine the intestinal bacterial composition of fecal samples collected at the end of every period, and the concentration of short-chain fatty acids and ammonia. A significant inhibition in fecal Escherichia coli and increase in Lactobacillus, Bifidobacterium, and Roseburia intestinalis were observed after consumption of LC01. Acetic acid and butyric acid were significantly higher in the probiotic stage and fecal ammonia was significantly lower. The results indicated a modulation effect of LC01 on the intestinal microflora of young adults, suggesting a beneficial effect on bowel health. LC01 may have potential value as a probiotic.