Title |
Lactobacillus paracasei subsp. paracasei LC01 Positively Modulates Intestinal Microflora in Healthy Young Adults |
Author |
Hao Zhang1,2, Jing Sun1, Xianting Liu1, Chuan Hong1, Yuanbo Zhu1, Aiping Liu3, Siqi Li1, Huiyuan Guo1, and Fazheng Ren1,2* |
Address |
1Beijing Laboratory for Food Quality and Safety, and Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, P. R. China, 2Beijing Higher Institution Engineering Research Center of Animal Product, Beijing, P. R. China, 3Diary Department, MengNiu Diary Company, Beijing, P. R. China |
Bibliography |
Journal of Microbiology, 51(6),777–782, 2013,
|
DOI |
10.1007/s12275-013-3279-2
|
Key Words |
human trial, Lactobacillus paracasei subsp. paracasei LC01, intestinal microflora, short-chain fatty acid, ammonia |
Abstract |
Lactobacillus paracasei subsp. paracasei LC01 (LC01) can
tolerate intestinal stresses and has antioxidant activity. To
evaluate the effect of the bacterium on human intestinal microflora,
a randomized, double-blind, placebo-controlled
human trial was carried out. Fifty-two healthy adult volunteers
were randomized equally to two groups. One group
consumed 12% (wt/vol) skimmed milk supplemented with
1010 CFU of LC01 each day for the 4-week treatment period,
and then consumed placebo in the next treatment period,
separated by a 2-week washout. The other group followed
the reverse order. Group-specific real-time PCR and biochemical
analyses was used to determine the intestinal bacterial
composition of fecal samples collected at the end of
every period, and the concentration of short-chain fatty acids
and ammonia. A significant inhibition in fecal Escherichia
coli and increase in Lactobacillus, Bifidobacterium, and Roseburia
intestinalis were observed after consumption of LC01.
Acetic acid and butyric acid were significantly higher in the
probiotic stage and fecal ammonia was significantly lower.
The results indicated a modulation effect of LC01 on the
intestinal microflora of young adults, suggesting a beneficial
effect on bowel health. LC01 may have potential value as a
probiotic. |