| Title | Antiviral Effect of Flavonol Glycosides Isolated from the Leaf of Zanthoxylum piperitum on Influenza Virus |
|---|---|
| Author | Song-Yi Ha1, Hana Youn2, Chang-Seon Song2, Se Chan Kang3, Jong Jin Bae4, Hee Tae Kim1, Kwang Min Lee1, Tae Hoon Eom1, In Su Kim1, and Jong Hwan Kwak1* |
| Address | 1School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea, 2College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea , 3Department of Life Science, Gachon University, Sungnam 461-701, Republic of Korea , 4Department of Oriental Pharmacy, Woosuk University, Jeonju 565-701, Republic of Korea |
| Bibliography | Journal of Microbiology, 52(4),340–344, 2014, |
| DOI | 10.1007/s12275-014-4073-5 |
| Key Words | Zanthoxylum piperitum, Rutaceae, flavonol glycoside, anti-influenza activity, neuraminidase inhibitor |
| Abstract | The ethanol extract of Zanthoxylum piperitum (L.) DC. showed in vitro antiviral activity against influenza A virus. Three flavonol glycosides were isolated from the EtOAc fraction of Z. piperitum leaf by means of activity-guided chromatographic separation. Structures of isolated compounds were identified as quercetin 3-O-β-D-galactopyranoside (1), quercetin 3-O-α-L-rhamnopyranoside (2), kaempferol 3-O-α-L-rhamnopyranoside (3) by comparing their spectral data with literature values. The anti-influenza viral activity of isolates was evaluated using a plaque reduction assay against influenza A/NWS/33 (H1N1) virus. The compounds also were subjected to neuraminidase inhibition assay in influenza A/NWS/33 virus. Compounds 1-3 exhibited antiviral activity against an influenza A virus in vitro, and inhibited the neuraminidase activity at relatively high concentrations. |