Title Comparison of JEV Neutralization Assay Using Pseudotyped JEV with the Conventional Plaque-Reduction Neutralization Test
Author Hee-Jung Lee 1, Kyung-Il Min 2, Ki Hoon Park 1, Hyo Jung Choi 1, Min-Kyoung Kim 1, Chi-Young Ahn 3, Young-Jin Hong 4, and Young Bong Kim 1*
Address 1Department of Bio-industrial technologies, Konkuk University, Seoul 143-701, Republic of Korea, 2Department of Biopharmaceuticals and Herbal Medicine Evaluation, National Institute of Food and Drug Safety Evaluation, Osong 363-951, Republic of Korea, 3Blood Products Team, National Institute of Food and Drug Safety Evaluation, Osong 363-951, Republic of Korea, 4Department of Pediatrics, College of medicine, Inha University, Incheon 402-751, Republic of Korea
Bibliography Journal of Microbiology, 52(5),435-440, 2014,
DOI DOI 10.1007/s12275-014-3529-y
Key Words Japanese encephalitis virus, neutralizing antibody, pseudovirus, PRNT
Abstract We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV- PV). JEV-PV-based neutralization assay offers several advan-tages compared with the current standard plaque-reduc-tion neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque re-duction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.