Title Altered mRNA Levels of MOV10, A3G, and IFN-α in Patients with Chronic Hepatitis B
Author Zhi-Wei Song1,2, Yan-Xiu Ma1, Li-Juan Fu1, Bao-qing Fu1, Xu Teng1, Si-Jia Chen1, Wei-Zhen Xu1, and Hong-Xi Gu1*
Address 1The Heilongjiang Key Laboratory of Immunity and Infection, Pathogenic Biology, Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang Province 150081, P. R. China, 2Laboratory Diagnosis Department, The First Affiliated Hospital of Harbin Medical University, 199 Xidazhi Street, Nangang District, Harbin 150001, P. R. China
Bibliography Journal of Microbiology, 52(6),510-514, 2014,
DOI 10.1007/s12275-014-3467-8
Key Words hepatitis B virus, innate immune, Moloney Leukemia Virus 10, APOBEC3G, IFN-α
Abstract To explore the relationship of the MOV10, A3G, and IFN-α mRNA levels with chronic hepatitis B virus (HBV) infection, Blood samples from 96 patients with chronic hepatitis B (CHB) and 21 healthy individuals as control were collected. HBV DNA load and aminotransferase in the serum were tested using real time PCR and velocity methods, respectively. The MOV10, A3G, and IFN-α mRNA levels in the peripheral blood mononuclear cells (PBMC) were examined through qRT-PCR. The MOV10, A3G, and IFN-α mRNA levels in CHB group was significantly lower than those in the control group (P<0.01, P<0.05, P<0.01, respectively). The A3G mRNA level in the high-HBV DNA load group was lower than that in the low-HBV DNA load group (P<0.05). However, no statistical difference was found in the MOV10 and IFN-α mRNA levels between the two HBV DNA load groups. Furthermore, the MOV10 mRNA level showed positive correlation with IFN-α in the control group. These results indicated that the expression of the innate immune factors MOV10, A3G, and IFN-α is affected by chronic HBV infection.